This standard describes the detection and isolation of Shiga toxin-producing Escherichia coli (STEC). The procedure includes the detection by Real Time PCR of the major virulence genes of STEC, stx1 and stx2 (Reference ) in enrichment cultures. Isolation of STEC from the enrichment culture is attempted if one or both stx genes are detected in the screening. This document is applicable to — products intended for human consumption, — products intended for animal feeding, — environmental samples in the area of food and feed production, handling, and — samples from the primary production stage.
Status: Under development
Technical Committee: ISO/TC 34/SC 9 Microbiology
- ICS :
- 07.100.30 Food microbiology
This standard contributes to the following Sustainable Development Goal:
ISO/CD 13136-1Stage: 30.60
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