Reference number
ISO 21115:2019
International Standard
ISO 21115:2019
Water quality — Determination of acute toxicity of water samples and chemicals to a fish gill cell line (RTgill-W1)
Edition 1
2019-04
Preview
ISO 21115:2019
69933
No disponible en español
Publicado (Edición 1, 2019)

ISO 21115:2019

ISO 21115:2019
69933
Idioma
Formato
CHF 173
Convertir Franco suizo (CHF) a tu moneda

Resumen

This document specifies a method for the determination of fish acute toxicity using the permanent cell line from rainbow trout (Oncorhynchus mykiss) gill, RTgill-W1. Cells in confluent monolayers in 24-well tissue culture plates are exposed to water samples, such as surface waters or different kinds of effluents, or to chemicals for 24 h and, thereafter, cell viability is assessed based on fluorescent cell viability indicator dyes (see 4.1). Data are then expressed as a percentage of unexposed control and toxicity quantified based on the percentage of cell viability versus the percentage of effluent or the chemical concentration in response curves (see Clause 9).

Informaciones generales

  •  : Publicado
     : 2019-04
    : Cierre de la revisión [90.60]
  •  : 1
     : 38
  • ISO/TC 147/SC 5
    13.060.70 
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