Reference number
ISO/TS 15216-2:2013
© ISO 2024
Technical Specification
ISO/TS 15216-2:2013
Microbiology of food and animal feed — Horizontal method for determination of hepatitis A virus and norovirus in food using real-time RT-PCR — Part 2: Method for qualitative detection
Edition 1
2013-03
Retirada
Retirada (Edición 1, 2013)

Resumen

ISO/TS 15216-2:2013 describes a method for qualitative detection of HAV and NoV genogroups I (GI) and II (GII), from test samples of foodstuffs or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR.

This approach is also relevant for detection of the target viruses on fomites, or of other human viruses in foodstuffs, on food surfaces or on fomites following appropriate validation and using target-specific primer and probe sets.

Informaciones generales

  • Estado
     : Retirada
    Fecha de publicación
     : 2013-03
    Versión corregida (en)
     : 2013-04
    Versión corregida (fr)
     : 2013-04
    Etapa
    : Retirada de la Norma Internacional [95.99]
  • Edición
     : 1
    Número de páginas
     : 27
  • Comité Técnico :
    ISO/TC 34/SC 9
    ICS :
    07.100.30 
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